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細(xì)胞倒置顯微鏡
Leica DMi8A電動(dòng)倒置熒光顯微鏡
Leica DMi8A是電動(dòng)型高端倒置熒光顯微鏡,既能完成常規(guī)細(xì)胞明場、相差對(duì)比、微分干涉對(duì)比(DIC)、調(diào)制對(duì)比(IMC)和熒光對(duì)比方法觀察,又可用于光轉(zhuǎn)化、光消融、鈣離子熒光等寬視場高速多通道熒光應(yīng)用研究。
設(shè)備名稱:倒置熒光顯微鏡 倒置熒光顯微成像系統(tǒng)
制 造 商:德國徠卡顯微系統(tǒng)公司(leica-microsystems) 產(chǎn) 地:德國
品 牌:徠卡/ Leica
型 號(hào): Leica DMi8 A Leica DMi8 C Leica DMi8 M
應(yīng)用配置方案見:【訂貨指南】
主要功能用途
Leica DMi8是一款采用先進(jìn)模塊化設(shè)計(jì),具有開放架構(gòu)、可靈活配置各種光學(xué)應(yīng)用模塊的研究級(jí)高端倒置熒光顯微鏡,與徠卡Leica Application Suite X (LAS X) 成像軟件相結(jié)合,既能完成常規(guī)細(xì)胞明場、相差對(duì)比、微分干涉對(duì)比(DIC)、調(diào)制對(duì)比(IMC)和熒光對(duì)比方法觀察與成像,又可用于光轉(zhuǎn)化、光消融、鈣離子熒光等寬視場高速多通道高級(jí)熒光應(yīng)用研究。
性能特點(diǎn)
DMi8多功能倒置熒光顯微鏡在光學(xué)接口設(shè)置、延時(shí)程序自動(dòng)聚焦、多色熒光信號(hào)同步采集、模塊化組合配置等方面的引入創(chuàng)新設(shè)計(jì),特別適合活細(xì)胞成像應(yīng)用。
1、智能化控制
可通過觸摸屏、功能控制元件或PC軟件實(shí)現(xiàn)完全智能自動(dòng)化和操作所有顯微鏡功能。
機(jī)身前部可選配傾角可調(diào)大尺寸彩色觸摸屏,為所有電動(dòng)和編碼功能的控制單元。操作界面友好直觀,可控制和查看顯微鏡各項(xiàng)工作狀態(tài):觀察方法、物鏡放大倍數(shù)及物鏡類型(干鏡、水鏡、油鏡),光源類型和光照強(qiáng)度,控制視場光闌及孔徑光闌、激發(fā)光濾塊轉(zhuǎn)換等。
當(dāng)選擇特定的對(duì)比度模式,DMi8A顯微鏡可自動(dòng)將所有組件移到光路中,將照明設(shè)置、對(duì)焦、光照強(qiáng)度和孔徑和視場光闌調(diào)整到該模式。
2、多Infinity Port光路接口
DMi8 顯微鏡光學(xué)擴(kuò)展模塊含有兩個(gè)新型無限遠(yuǎn)光路接口(Infinity Port)。主機(jī)的左側(cè)、右側(cè)、底部及目鏡筒共配備有4個(gè)相機(jī)接口。
新增的入射光路接口,有助于整合額外的光源和激光系統(tǒng)。如Infinity 光操作系統(tǒng)、Infinity TIRF、FRAP、光轉(zhuǎn)換等先進(jìn)顯微技術(shù)所需激發(fā)光源,實(shí)現(xiàn)FRAP、光活化、激光消融、激光鑷等新型顯微技術(shù)應(yīng)用。
3、快速熒光成像高速圖像采集領(lǐng)域
對(duì)于高速成像,徠卡DMi8集成外部熒光轉(zhuǎn)換功能(External Filter Wheel, EFW)、自動(dòng)熒光照明強(qiáng)度管理系統(tǒng)(Fluorescence Intensity Manager, FIM),微秒快速LED光源,使得DMi8 sequencer可以在微秒級(jí)別速度和精度下同時(shí)控制,實(shí)現(xiàn)快速、準(zhǔn)確地?zé)晒獬上瘛S兄诓蹲交罴?xì)胞的動(dòng)態(tài)過程,將光毒性以及光漂白降至最低。
利用LAS X 和Dual-View 圖像分割器,同時(shí)獲取多通道圖像。
專用FURA2成像系統(tǒng),物鏡對(duì)340nm處的UV透射高達(dá)60%,配合極快的EFW轉(zhuǎn)換功能(24ms切換時(shí)間),足以捕捉如活細(xì)胞內(nèi)鈣離子流量快速動(dòng)態(tài)測定過程。
5、寬視場成像
DMi8標(biāo)配FOV 19 mm視場攝像頭端口、大幅面 sCMOS 相機(jī),極大拓展了圖像視野。
4、自適應(yīng)調(diào)焦控制
利用 LED 光束輔助功能的徠卡自適應(yīng)調(diào)焦控制(Adaptive Focus Control, AFC)和閉環(huán)聚焦(closed-loop focus)調(diào)焦控制系統(tǒng),只需在Leica LAS X軟件單擊一次激活自動(dòng)對(duì)焦以及閉環(huán)調(diào)焦按鈕,Leica DMi8 調(diào)焦驅(qū)動(dòng)器以 20 nm 的重定位精度實(shí)現(xiàn)自動(dòng)實(shí)現(xiàn)精確地保持焦平面。即使在活細(xì)胞延時(shí)實(shí)驗(yàn)(timelapse experiments)中因?qū)嶒?yàn)體系出現(xiàn)劇烈溫度變化、載物臺(tái) x-y 移動(dòng),物鏡焦平面也始終保持穩(wěn)定。AFC完全集成到 LAS X軟件中,并可與基于圖像的自動(dòng)對(duì)焦系統(tǒng)結(jié)合使用,典型應(yīng)用是活細(xì)胞成像、孔板篩選、多位置實(shí)驗(yàn)和比率成像。
6、模塊化配置
DMi8 采用模塊化設(shè)計(jì),從手動(dòng)到電動(dòng)各種組件可自由組合,以適應(yīng)不同的透射光方法和熒光應(yīng)用方法。相應(yīng)編碼組件或電動(dòng)組件的設(shè)置狀態(tài)可自動(dòng)從顯微鏡傳輸?shù)竭B接的PC(USB)上的軟件,作為圖像信息要素被記錄保存。
DMi8的可選組件包括:
電動(dòng)或編碼透射臂,帶各種聚光鏡系統(tǒng)
電動(dòng)或手動(dòng)入射光軸
多種照明LED 照明、EL6000或鹵素?zé)粽彰鞴庠聪到y(tǒng)
電動(dòng)或手動(dòng)對(duì)焦驅(qū)動(dòng)(可選擇手動(dòng)2速或3速)
電動(dòng)或編碼放大倍率轉(zhuǎn)換器(Magnification Changer)
電動(dòng)或編碼物鏡轉(zhuǎn)換器(6個(gè)M25或M32鏡頭)
用于照明調(diào)節(jié)電動(dòng)或編碼6孔(2孔)反射立方體支架(reflector Turret)
DIC棱鏡的電動(dòng)或編碼變化
配置電動(dòng)側(cè)向攝像頭輸出;
高速線性電動(dòng)載物臺(tái)(提供精確定位,可在40x物鏡下每秒定位5個(gè)觀察位置。振動(dòng)傳感器可確保載物臺(tái)在圖像采集過程中完全靜止。圖像采集與載物臺(tái)移動(dòng)之間的同步實(shí)現(xiàn)快速、高清晰度圖像的采集)。
主要技術(shù)規(guī)格
Leica DMi8多功能倒置顯微鏡主機(jī)分為手動(dòng)型DMi8 Manual(DMi8 with manual components)、編碼型DMi8 Coded(DMi8 with encoded components)、電動(dòng)型DMi8 Automated(DMi8 with motorized components)三款。區(qū)別在于工作光學(xué)組件、照明光軸、光路接口的手動(dòng)或電動(dòng)控制。
型號(hào) | Leica DMi8 M | Leica DMi8 C | Leica DMi8 A | |
顯微鏡鏡座(Base Stand) | 內(nèi)置(Internal) 供電單元 | Electronic box CTR basic Electronic box CTR advanced | ||
顯示方式 | NA | 6個(gè) or 12 個(gè)LED指示燈 | 可選LED燈 | |
機(jī)身側(cè)面控制按鈕(Function keys) | ||||
觸摸屏Touchscreen | ||||
聚焦方式 | 手動(dòng)粗細(xì)兩檔調(diào)焦(Manual 2-gear) | 電動(dòng)調(diào)焦 | ||
NA | 手動(dòng)粗/細(xì)/止動(dòng)三檔調(diào)焦及調(diào)焦旋鈕張力調(diào)整( Manual 3-gear with focus stop and torque adjustment) | |||
物鏡轉(zhuǎn)盤 (Objective Turret) | 手動(dòng)6孔M25轉(zhuǎn)盤 | 手動(dòng)編碼6孔M32轉(zhuǎn)盤(coded) 手動(dòng)編碼6孔M25轉(zhuǎn)盤(coded) | 電動(dòng)6孔M32轉(zhuǎn)盤(motorized) 手動(dòng)編碼6孔M32轉(zhuǎn)盤 電動(dòng)6孔M25轉(zhuǎn)盤(motorized) 手動(dòng)編碼6孔M25轉(zhuǎn)盤 | |
反射器轉(zhuǎn)盤 (Reflector Turret) | 固定明場分光器(Fixed BF-splitter) | 2孔編碼反光器轉(zhuǎn)盤(coded) 6孔編碼反光器轉(zhuǎn)盤(coded) 6孔反光器轉(zhuǎn)盤 | 6孔電動(dòng)反射器轉(zhuǎn)盤 2孔編碼反射器轉(zhuǎn)盤 6孔編碼反射器轉(zhuǎn)盤 6孔手動(dòng)反射器轉(zhuǎn)盤 | |
反射光光軸 (Reflected light axis) | NA | NA | 電動(dòng)光軸,含: 可調(diào)中視場光闌(field diaphragm) 孔徑針孔照明(aperture pinhole illumination) 偏振器(polarizer) 透射光濾光片滑塊(filter slider)插槽 | |
手動(dòng)光軸,含: 可調(diào)中視場光闌(field diaphragm)和孔徑光闌(aperture iris diaphragm) 手動(dòng) UC-3D 照明 偏振器(polarizer)和附加透射光濾光片滑塊(filter slider)插槽 | ||||
對(duì)比觀察技術(shù)(Contrasting techniques) | ||||
入射光(Incident light, IL) | BF, UC-3D, DIC, Pol | BF, UC-3D, HDF, DIC, Pol, Fluo | BF, UC-3D, HDF, DIC, Pol, Fluo | |
透射光(Transmitted light, TL) | NA | BF, Pol, Ph, DIC, DF | BF, Pol, Ph, DIC, DF | |
變倍器(Magnification changer) | NA | 前置端口激活 手動(dòng)編碼 1.5x或2.0x可選 | 所有端口激活 電動(dòng)操作 1.5x或2.0x可選 | |
操作控制元件(Control Elements) | ||||
主機(jī)基座控制功能 | 光強(qiáng)度控制 | 取決于配置 | ||
NA | IL/TL轉(zhuǎn)換 | 前置面板控制按鈕 | ||
NA | NA | 觸摸屏 | ||
NA | NA | 左側(cè):照明和對(duì)比度管理器 | ||
NA | NA | 右側(cè):可編程功能按鈕 | ||
聚焦控制 | ||||
Smart Move控制組件 | NA | NA | 用于對(duì)焦和載物臺(tái)移動(dòng)的控制元件 | |
4 個(gè)可自由編程的功能鍵 | ||||
STP4000 | NA | NA | 外置 6 英寸觸摸屏 | |
NA | NA | 帶 xyz 控制的外部 6 英寸觸摸屏 | ||
STP8000 | NA | NA | 外置6寸觸摸屏,帶 xyz 控制 11 個(gè)可自由編程功能鍵 | |
載物臺(tái)(Stage) | 固定載物臺(tái)帶不同插件、樣品導(dǎo)軌(object guide) | 電動(dòng)3板載物臺(tái)(Motorized 3-plate stage) | ||
NA | NA | 電動(dòng)掃描臺(tái)(Scanning stage )帶插件 | ||
手動(dòng)三板載物臺(tái)(Manual 3-plate stage)及插件 | ||||
帶阻尼滑動(dòng)臺(tái)(Gliding stage) | ||||
照明(Illumination) | LED冷光源 | |||
鹵素?zé)?(Halogen Bulb) (外置電源) | ||||
NA | EL6000水銀金屬鹵素?zé)艄庠?/span> | |||
NA | Hg 100W汞燈(外置電源) | |||
應(yīng)用軟件 | Leica Application Suite X (LAS X) | |||
安裝運(yùn)行條件
工作環(huán)境溫度:15℃ ~ 35℃;
工作外部電源:AC 100-240V AC, 50/60Hz
相對(duì)濕度范圍:max 90% R.H. @ 30℃,無冷凝
組成配置
Leica DMi8A、DMi8C、DMi8M三款倒置顯微鏡中,Leica DMi8A的透射光軸(Transmitted-Light Axis)、入射熒光軸(Incident Light Axis)、光學(xué)載體(Optic Carrier)、熒光濾塊轉(zhuǎn)盤(Reflector Turret)、物鏡轉(zhuǎn)盤(Nosepiece)、載物臺(tái)即可用配置全電動(dòng)工作組件,也兼容帶編碼的各種工作組件。DMi8C則適用于裝配編碼型器件工作,而DMi8M則只能配置全手動(dòng)控制型組件。
細(xì)胞生物學(xué)實(shí)驗(yàn)中,因觀察對(duì)比方法、研究手段的不同,入射照明光源、入射光軸、光學(xué)載體元件、熒光濾塊、物鏡配置、圖像光路出口布局、載物臺(tái)及外圍控制設(shè)備配置要求不同。徠卡為電動(dòng)、編碼、手動(dòng)主機(jī)分別提供了具有不同無限遠(yuǎn)光學(xué)成像輸出口(camera ports)設(shè)置的基座(Base Stand)。主體基座根據(jù)應(yīng)用需求,需添加入射光軸(incident light axis)、透射光臂(transmitted light arms)、LED透射光源、聚光鏡(Condensers)套件、DIC/相差/IMC/前端模塊(front modules)/放大倍數(shù)轉(zhuǎn)換器(magnification changers)、熒光照明光源、熒光濾片立方模塊、物鏡調(diào)焦控制、物鏡轉(zhuǎn)盤、載物臺(tái)及驅(qū)動(dòng)控件、目鏡筒、數(shù)碼攝像頭等搭建相應(yīng)的應(yīng)用配置系統(tǒng)。
除電動(dòng)型DMi8A主機(jī),需配置電控制元件(Control-Elements)。DMi8C型主機(jī)還需在機(jī)身正面根據(jù)編碼元件配置種類,增配相應(yīng)LED指示和控制按鈕。
Leica Assistant LAS Store & Recall Tube(12730488),即Leica LAS Store and Recall Module,徠卡顯微鏡助手、徠卡LAS軟件存儲(chǔ)和調(diào)用功能模塊,會(huì)自動(dòng)將生成每張圖所設(shè)定的照明強(qiáng)度和對(duì)比度方法、放大倍率和曝光時(shí)間等設(shè)置直接與圖像一起存儲(chǔ)。只需在圖像上單擊一次鼠標(biāo),可快速恢復(fù)所有顯微鏡設(shè)置,確保相同實(shí)驗(yàn)中所有所獲取圖像成像條件完全相同。便于工業(yè)和生命科學(xué)應(yīng)用中重新檢查比較樣品,無需在實(shí)驗(yàn)室中通過手工記錄和人工記錄這些顯微鏡設(shè)置要素。
下表列出了部分常用配置組件。
品名 | 品牌 | 貨號(hào) | 規(guī)格 | 描述 | 報(bào)價(jià) |
電動(dòng)入射光軸 | Leica | 11889090 | Each | T-Haus IND mot. , IL/TL | 詢價(jià) |
電動(dòng)LED光源 | Leica | 11525103 | Each | LED lamp houses wit TTL-shutter 185 cm cable,for mot. Stage connected via CTR box | 詢價(jià) |
手動(dòng)入射光軸 | Leica | 11889089 | Each | T-Haus IND man. (IL axis man.) | 詢價(jià) |
手動(dòng)LED光源 | Leica | 11525101 | Each | LED lamp houses with TTL-shutter, 70 cm cable | 詢價(jià) |
電動(dòng)可傾斜透射照明臂 | Leica | 11525111 | Each | Tilting motorized transmitted light axis with Field Diaphragm | 詢價(jià) |
手動(dòng)可傾斜透射照明臂 | Leica | 11525107 | Each | Tilting manual transmitted light axis | 詢價(jià) |
電動(dòng)S28聚光鏡座 | Leica | 11525372 | Each | Motorized Condenser S28 base with fixed mount | 詢價(jià) |
S28/0.55 聚光鏡頭 | Leica | 11505234 | Each | Condenser head S28/0.55 | 詢價(jià) |
S28聚光鏡相差組件 | Leica | 11521505 | Each | Light ring set S23/0.53/S28/0.55/S40/0.4 | 詢價(jià) |
電動(dòng)起偏器 | Leica | 11522120 | Each | S28 Motorized polarizer holder including polarizer | 詢價(jià) |
IMC滑塊 | Leica | 11522075 | Each | IMC slider for objectives w. pupil B or C | 詢價(jià) |
電動(dòng)S70/0.30套件 | Leica | 11525370 | Each | Motorized condenser S70 base incl. head S70/0.30 | 詢價(jià) |
電動(dòng)S70相差組件 | Leica | 11521506 | Each | Light ring set for phase contrast S70/0.30 | 詢價(jià) |
電動(dòng)S70起偏器 | Leica | 11522120 | Each | S70 Motorized polarizer holder including polarizer | 詢價(jià) |
電動(dòng)S7相差組件 | Leica | 11522090 | Each | Light ring set for phase contrast S70/0.30 | 詢價(jià) |
DIC棱鏡適配器 | Leica | 11522093 | Each | 32/43 mm adapter for Wollaston prisms | 詢價(jià) |
S70/0.30手動(dòng)聚光鏡 | Leica | 11525089 | Each | Manual S70/0.30 Condenser base with fixed condenser head | |
手動(dòng)S70相差套件 | Leica | 11522090 | Each | Light ring set for phase contrast S70/0.30 | 詢價(jià) |
手動(dòng)S70起偏器 | Leica | 11522094 | Each | Manual polarizer in round insert | 詢價(jià) |
手動(dòng)S70 DIC棱鏡適配器 | Leica | 11522093 | Each | manual S70 condensers turrets 32/43 adapter for each prisms (max 3) | 詢價(jià) |
電動(dòng)6孔熒光物鏡轉(zhuǎn)盤 | Leica | 11889022 | Each | Motorized 6-position Fluorescence Turret | 詢價(jià) |
手動(dòng)6孔熒光物鏡轉(zhuǎn)盤 | Leica | 11889020 | Each | Manual 6-position reflector turret | 詢價(jià) |
FITC熒光濾塊 | Leica | 11525307 | Each | Filter Cubes FITC Filter Diameter 25 mm, Excitation Filters 480/30nm Dichroic Filters 505nm, Barrier Filters 535/45nm | 詢價(jià) |
DAPI熒光濾塊 | Leica | 11525304 | Each | Filter Cubes DAPI Filter Diameter 25 mm, Excitation Filters 375/28nm Dichroic Filters 415nm, Barrier Filters 460/60nm | 詢價(jià) |
GFP熒光濾塊 | Leica | 11525314 | Each | Filter Cubes GFP Filter Diameter 25 mm, Excitation Filters 470/40nm Dichroic Filters 500nm, Barrier Filters 535/50nm | 詢價(jià) |
Texas Red熒光濾塊 | Leica | 15525310 | Each | Filter Cubes Texas Red Filter Diameter 25 mm, Excitation Filters 560/40nm Dichroic Filters 595nm, Barrier Filters 630/60nm | 詢價(jià) |
外置電動(dòng)熒光光軸 | Leica | 11889053 | Each | Motorized external fluorescence-axis The module includes: internal filter wheel (IFW) light fiber connection motorized diaphragms slot for Leica fast external filter wheels | 詢價(jià) |
集成手動(dòng)熒光光軸 | Leica | 11889038 | Each | DMi8 T-House(2) and integrated manual Fluorescence Axis with integrated manual fluorescence module | 詢價(jià) |
手動(dòng)熒光強(qiáng)度調(diào)節(jié)器 | Leica | 11525205 | Each | Manual attenuator with 5 positions for T-House(2) with integrated manual Fluorescence Axis | 詢價(jià) |
EL 6000熒光激發(fā)光源 | Leica | 11504115 | Each | External light source EL 6000 | 詢價(jià) |
EL6000傳輸光線 | Leica | 11504116 | Each | Gel fiber-optics, 2m | 詢價(jià) |
光線接頭 | Leica | 11504117 | Each | 1-inch fiber optics adapter for lamp mounts | 詢價(jià) |
固定角度雙目鏡筒 | Leica | 11889025 | Each | Bino FixTube without Bertrand lens | 詢價(jià) |
角度可調(diào)雙目鏡筒 | Leica | 11889026 | Each | Bino ErgoTube without Bertrand lens | 詢價(jià) |
帶勃氏透鏡可調(diào)雙目鏡筒 | Leica | 11889031 | Each | Bino ErgoTube with Bertrand lens | 詢價(jià) |
三目可調(diào)鏡筒 | Leica | 11889028 | Each | Trinocular ergonomic tube with side camera port variable light path, 100%/0% | 詢價(jià) |
帶勃氏透鏡三目鏡筒 | Leica | 11889027 | Each | Trino ErgoTubes 100/0 with Bertrand lens | 詢價(jià) |
10x/22目鏡 | Leica | 11507807 | each | Eyepiece HC PLAN s 10x/22 Br. M | 詢價(jià) |
10x/25目鏡 | Leica | 11507808 | each | Eyepiece HC PLAN s 10x/25 Br. M | 詢價(jià) |
帶目鏡微尺10x/22目鏡 | Leica | 11557805 | Each | Eyepiece HC PLAN s 10x/22 Br.M +reticule | 詢價(jià) |
帶目鏡微尺10x/25目鏡 | Leica | 11557806 | Each | Eyepiece HC PLAN s 10x/25 Br.M +reticule | 詢價(jià) |
電動(dòng)機(jī)身左相機(jī)接口 | Leica | 11889046 | Each | Motorized sideport, 100/0, left | 詢價(jià) |
手動(dòng)機(jī)身左相機(jī)接口 | Leica | 11889043 | Each | Side Port left 100/0, manual | 詢價(jià) |
M32 6孔物鏡電動(dòng)轉(zhuǎn)盤 | Leica | 11889095 | Each | Motorized Nosepiece M32 Motorized coded 6-fold objective nosepiece with M32 mm threads and 45 mm parfocal distance. | 詢價(jià) |
M25 6孔物鏡電動(dòng)轉(zhuǎn)盤 | Leica | 11889050 | Each | Motorized Nosepiece M25 Motorized coded 6-fold objective nosepiece with M25 mm threads and 45 mm parfocal distance. | 詢價(jià) |
Z軸電動(dòng)聚焦控制 | Leica | 11889055 | Each | Motorized focus system with 12 mm travel range. | 詢價(jià) |
Z軸閉環(huán)聚焦控制 | Leica | 11889056 | Each | Motorized closed loop focus system with encoded 12 mm travel range. | 詢價(jià) |
M32 6孔物鏡手動(dòng)轉(zhuǎn)盤 | Leica | 11889094 | Each | Manual Coded Nosepiece M32 Coded 6-fold objective nosepiece with M32 mm threads and 45 mm parfocal distance. | 詢價(jià) |
M25 6孔物鏡手動(dòng)轉(zhuǎn)盤 | Leica | 11889049 | Each | Manual Coded Nosepiece M25 Coded 6-fold objective nosepiece with M25 mm threads and 45 mm parfocal distance. | 詢價(jià) |
焦距引導(dǎo)盒 | Leica | 11889116 | Each | Focus guide box | 詢價(jià) |
手動(dòng)3檔聚焦旋鈕 | Leica | 11889100 | Each | Manual Focus 3-gear (coarse/medium/fine) with torque adjustment and adjustable upper focus stop. | 詢價(jià) |
電動(dòng)3板載物臺(tái) | Leica | 11525386 | Each | Motorized 3-plate-stage (without insert) | 詢價(jià) |
手動(dòng)3板載物臺(tái) | Leica | 11525387 | Each | Manual 3-plate-stage (without insert) | 詢價(jià) |
圓形通光插件固定架 | Leica | 11525379 | Each | Mounting frame 160x130 for round insert | 詢價(jià) |
20mm直徑通光孔插件 | Leica | 11525380 | Each | Insert with 20 mm inside diameter | 詢價(jià) |
CTR電動(dòng)控制單元 | Leica | 11525207 | Each | Leica CTR advanced box for Leica DMi8 A | 詢價(jià) |
電動(dòng)載物臺(tái)驅(qū)動(dòng)器 | Leica | 11525210 | Each | CTR Board xy basic,15 pins,to control 3-plate motor stages | 詢價(jià) |
SmartMove遙控手柄 | Leica | 11525115 | Each | Leica SmartMove Remote Control Module for DM/DMI series | 詢價(jià) |
DFC3000G數(shù)碼相機(jī) | Leica | 11547005 | Each | Leica DFC3000 G Digital monochrome, passive-cooled camera Sony ICX445 interline transfer CCD sensor 1.3 MP Pixel; 3.75 μm × 3.75 μm pixel size Exposure time 7 μsec – 5 sec Bit depth 8 bit/12 bit/16 bit | 詢價(jià) |
C-mount數(shù)碼相機(jī)接口 | Leica | 11541544 | Each | C-mount adapter 0.55x HC for 1/2-inch chip cameras without zoom magnification. | 詢價(jià) |
基本耗材
(略)
訂貨指南
Leica DMi8系列倒置熒光顯微鏡,具有開放型架構(gòu),視具體應(yīng)用需求不同,各功能模塊采用積木式組合配置。
DMi8A采用電動(dòng)工作組件,可通過機(jī)身正面觸摸屏或聯(lián)機(jī)Leica Application Suite X應(yīng)用軟件,鏡頭聚焦、光路轉(zhuǎn)換、光源強(qiáng)度調(diào)整、快門關(guān)閉、物鏡及光學(xué)元件的轉(zhuǎn)入/轉(zhuǎn)出光路全自動(dòng)完成,操作簡化,并可減少人為操作失誤。同時(shí),系統(tǒng)自動(dòng)將成像所涉及光學(xué)元件、光路設(shè)置信息整合到所生成圖像文件中,用戶可隨時(shí)查看和調(diào)用圖像生成所需的各項(xiàng)設(shè)置、條件參數(shù)信息,確??稍谙嗤瑮l件下獲得高質(zhì)量細(xì)胞圖像。
DMi8A的具有左、右、上、底部四路光學(xué)圖像輸出口,數(shù)碼相機(jī)既可以采用三目鏡筒安裝,也可以安裝與機(jī)身左、右側(cè)。系統(tǒng)支持多相機(jī)組合應(yīng)用方案,分別為不同檢測通路分配專用圖像采集光路。系統(tǒng)可根據(jù)預(yù)先設(shè)置,實(shí)現(xiàn)多路圖像信號(hào)的實(shí)時(shí)同步采集。
對(duì)于Fura2、FRET和光轉(zhuǎn)化等多光路、同步熒光圖像采集分析應(yīng)用,為實(shí)現(xiàn)不同熒光檢測通道信號(hào)的快速切換,DMi8A可引入電動(dòng)控制的Leica EL6000光源及快速濾光片輪(External Filter Wheel, EFW)相結(jié)合,配合專用濾光片模塊,可實(shí)現(xiàn)24ms完成熒光通道的超快速切換,實(shí)現(xiàn)高達(dá)40fps的圖像采集傳輸性能。
利用DMi8具有的雙Infinity-Port照明光口配置(對(duì)應(yīng)于T-house 3),DMi8A可添加TIRF功能所需的激發(fā)光源模塊及TRIF專用物鏡,構(gòu)建TRIF分析平臺(tái)。
本欄目僅列出了DMi8A、DMi8C、DMi8M在常規(guī)細(xì)胞顯微操作、活細(xì)胞相差觀察、細(xì)胞熒光成像等基礎(chǔ)型應(yīng)用的配置參考方案。更多應(yīng)用配置方案及細(xì)節(jié),請(qǐng)洽客服。
序號(hào) | 品名 | 貨號(hào) | 套裝配置描述 | 報(bào)價(jià) |
1-1 | DMi8A PH/ FL應(yīng)用配置套裝 | 11889122-PF | DMi8 A Microscope PH/FL applications package Configurations Including: 11889122 Leica DMi8 A with Motorized Side Port 11889032 optical carrier w/o IMC 11889024 Mot. mag. Changer, incl. Fixed 1x tube lens 11888699 1.5x mag.changer 11889090 Motorized incident light axis 11525102 LED lamp house w/o Shutter, long cable 11525111 Tilting motorized transmitted light axis 11521582 Panchromatic green filter 11525372 Condenser Base 1-40 for fixed lens,motor 11505234 S28/0.55 Condenser head 11521505 Light ring set for PH and DF contrast S23–S28 11889052 motorized external Fluorescence axis 11504115 External light source EL 6000 11504116 Liquid Light Guide for EL6000 11889022 6-fold Motorized Fluorescence turret 11525304 Filter Cubes DAPI 11525314 Filter Cubes GFP 15525310 Filter Cubes Texas Red 11889031 Bino ErgoTube with Bertrand lens 11507808 Eyepiece HC PLAN S 10x/25 Br.M 11557806 Eyepiece HC PLAN s 10x/25 Br.M +reticule 11888259 Side port prism, 100% left 11889095 Motorized Nosepiece 6x M32 11889055 Z-Focus, motorized focus 11889056 Z-Focus, closed loop focus 11506303 Obj. N PLAN 5x/0.12 PH0 11506406 Obj. N PLAN 10x/0.25 PH1 11506248 Obj. N PLAN L 20x/0.35 PH1 11506298 Obj. N PLAN L 40x/0.55 CORR PH2 11525386 Motorized 3-plate stage 11525379 Mounting frame for round insert 11525380 Round insert with 20mm opening 11525207 CTR Box advanced 11525210 CTR board XY basic 11525115 SmartMove Remote Control Module 11547110 Leica DFC7000 GT CCD Camera 11541543 0.7x C-mount adapter HC | 詢價(jià) |
1-2 | DMi8A IMC/ FL應(yīng)用配置套裝 | 11889122-MF | DMi8 A Microscope IMC-FL applications package Configurations Including: 11889122 Leica DMi8 A with Motorized Side Port 11889024 Mot. mag. Changer, incl. Fixed 1x tube lens 11888699 1.5x mag.changer 11889033 Optic carrier for IMC 11888384 Front module for IMC 11889090 Motorized incident light axis 11525102 LED lamp house w/o Shutter, long cable 11525111 Tilting motorized transmitted light axis 11521582 Panchromatic green filter 11525372 Condenser Base 1-40 for fixed lens,motor 11505234 S28/0.55 Condenser head 11522075 IMC slider for objectives w. pupil B or C 11889052 motorized external Fluorescence axis 11504115 External light source EL 6000 11504116 Liquid Light Guide for EL6000 11889022 6-fold Motorized Fluorescence turret 11525304 Filter Cubes DAPI 11525314 Filter Cubes GFP 15525310 Filter Cubes Texas Red 11889031 Bino ErgoTube with Bertrand lens 11507808 Eyepiece HC PLAN S 10x/25 Br.M 11557806 Eyepiece HC PLAN s 10x/25 Br.M +reticule 11888259 Side port prism, 100% left 11889095 Motorized Nosepiece 6x M32 11889055 Z-Focus, motorized focus 11889056 Z-Focus, closed loop focus 11506302 Obj. N PLAN 5x/0.12 11506400 Obj. HC FL PLAN 10x/0.25 11506519 Obj. HC PL FLUOTAR 20x/0.55 11506297 Obj. N PLAN L 40x/0.55 CORR 11525386 Motorized 3-plate stage 11525379 Mounting frame for round insert 11525380 Round insert with 20mm opening 11525207 CTR Box advanced 11525210 CTR board XY basic 11525115 SmartMove Remote Control Module 11547110 Leica DFC7000 GT CCD Camera 11541543 0.7x C-mount adapter HC | 詢價(jià) |
2-1 | DMi8C DF/DIC/PL/FL應(yīng)用配置套裝 | 11510055 | DMi8 C for BF/DF/DIC/PL/FL application package Configurations Including: 11889117 DMi8 C manual coded microscope base Stand 11889089 manual incident light axis T-house(6) 11889061 operation (front) 12x LED 11889067 control (l) light+stop 11889094 nosepiece, 6-way, M32, coded 11889021 fluorescence turret, 6-way, coded 11889100 Manual focusing drive, 3-speed 11889116 drive box (Focus guide box) 11889032 optical carrier 12730488 Microscope Assistant LAS Store & Recall 11889026 Bino Ergo tube 11507807 Pair of eyepieces HC PLAN s 10x/22 Br. M 11525100 LED without shutter and short cable 11565053 Reflector cube BF 11565054 Reflector cube DF 11525375 Reflector cube ICR 11889043 Side port, left 100/0 11525387 Manual 3-plate table 100x50 mm 11525379 insert frame 160x130 for round use 11525381 Round insert with 10mm opening 11525380 Round insert with 20mm opening Lenses 11566046 Obj. HC PL FL 5x/0.15 BD 11566503 Obj. HC PL FL 10x/0.30 BD 11566509 Obj. HC PL FLUOTAR 20x/0.45 BD Objective Prisms IC-Prism | 詢價(jià) |
2-2 | DMi8 C 工業(yè)檢測應(yīng)用套裝 | 11510050 | DMi8 C for BF/DF applicaton package Configurations Including: 11889117 DMi8 C manual, coded microscope base Stand 11889089 manual incident light axis T-house (6) 11889063 control (front) 6x LED 11889067 control (l) light+stop 11889094 nosepiece, 6x, M32, coded 11889098 filter turret, 2-way, coded 11889100 Manual focusing pinion, 3-speed 11889116 drive box 11889032 optical carrier 12730488 Microscope Assistant LAS Store & Recall 11889026 Tube, Bino Ergo 11507807 Pair of eyepieces HC PLAN s 10x/22 Br. M 11525100 LED without shutter and short cable 11565053 Reflector cube BF 11565054 Reflector cube DF 11889043 Side port left 100%/0% 11525387 Manual 3-plate table 100x50 mm 11525379 insert frame 160x130 for round 11525381 round Insert with 10mm aperture 11525380 Round insert with 20mm aperture Lenses 11566067 Obj. N PLAN EPI 20x/0.40 BD 11566077 Obj. N PLAN EPI 5x/0.12 BD 11566083 Obj. N PLAN EPI 10x/0.25 BD | 詢價(jià) |
3-1 | DMi8 M 熒光顯微成像應(yīng)用套裝 | 11889092-FL | DMi8 M Microscope PH/FL applications package Configurations Including: 11889092 DMi8 M basic stand BF only 11521582 Panchromatic green filter 11525373 Condenser Base 1-40 for fixed lens, coded 11505234 S28/0.55 Condenser head 11521505 Light ring set for PH and DF contrast S23–S28 11889038 T-House(2) with integrated man fluor-axis 11525205 Attenuator for T-House(2), manual FIM 11504115 External light source EL 6000 11504116 Liquid Light Guide for EL6000 11504117 Lightguide coupler 1" 11889020 6-fold manual Fluorescence Turret 11525304 Filter Cubes DAPI 11525314 Filter Cubes GFP 15525310 Filter Cubes Texas Red 11889031 Bino ErgoTube with Bertrand lens 11507808 Eyepiece HC PLAN S 10x/25 Br.M 11557806 Eyepiece HC PLAN s 10x/25 Br.M +reticule 11889043 Manual left camera side port 100/0 11506303 Obj. N PLAN 5x/0.12 PH0 11506406 Obj. N PLAN 10x/0.25 PH1 11506248 Obj. N PLAN L 20x/0.35 PH1 11506298 Obj. N PLAN L 40x/0.55 CORR PH2 11525387 Manual 3-plate stage 11525379 Mounting frame for round insert 11525380 Round insert with 20mm opening 11547110 Leica DFC7000 GT CCD Camera 11541543 0.7x C-mount adapter HC | 詢價(jià) |
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