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伯樂Western Blot蛋白電轉(zhuǎn)槽
伯樂Mini-Trans Blot小型蛋白電轉(zhuǎn)印槽
伯樂Mini-Trans Blot 小型蛋白凝膠電轉(zhuǎn)印槽(1703930),即WB電轉(zhuǎn)儀、Western Blot轉(zhuǎn)膜儀。本欄目提供了Mini-Trans Blot 工作組件和常規(guī)Western Blot實驗平臺的選型配置方案指南。
設(shè)備名稱:蛋白電轉(zhuǎn)儀 WB電轉(zhuǎn)印槽 濕式轉(zhuǎn)印槽 轉(zhuǎn)膜槽
制 造 商:美國伯樂(Bio-Rad)公司 產(chǎn) 地:中國
品 牌:伯樂(Bio-Rad)
型 號:Mini-Trans Blot 配置方案:見【訂貨指南】
主要功能用途
蛋白電轉(zhuǎn)印槽,習(xí)稱轉(zhuǎn)膜槽、轉(zhuǎn)膜儀。伯樂Mini Trans-Blot電轉(zhuǎn)印槽可把電泳分離后的蛋白質(zhì)或核酸從聚丙烯酰胺等凝膠中轉(zhuǎn)印至如PVDF膜、硝酸纖維素膜等支持載體上。提供大分子量跨度范圍提供蛋白高效而穩(wěn)定轉(zhuǎn)印。Mini Trans-Blot能同時容納2個小型凝膠轉(zhuǎn)印電極,快速高質(zhì)量地轉(zhuǎn)印2塊小型凝膠,主要用于實驗室蛋白印記分析(Western Blotting)和核酸轉(zhuǎn)印(Northern Bloting\Southern Blotting)分析。
性能特點
伯樂Mini-Trans Blot小型電轉(zhuǎn)槽工作時的電壓設(shè)置、轉(zhuǎn)印時間和冷卻方法,均可根據(jù)樣品屬性靈活選擇。
可在1小時內(nèi)完成2塊10×7.5 cm小型凝膠轉(zhuǎn)印,也可以進(jìn)行低強度隔夜轉(zhuǎn)印。
采用電極絲,相距4cm ,產(chǎn)生強電場,產(chǎn)熱少。
彩色標(biāo)記的轉(zhuǎn)印夾和電極確保轉(zhuǎn)印過程中凝膠的正確定向。
藍(lán)色致冷芯可完全置于小型Trans-Blot 轉(zhuǎn)印槽內(nèi),在快速轉(zhuǎn)印過程中吸收熱量。
Mini-Trans Blot電轉(zhuǎn)槽與Mini-PROTEAN Tetra cells (1658001)垂直電泳槽兩套流程,所用的緩沖液槽組件完全兼容,可互換。
主要技術(shù)規(guī)格
伯樂Western Blot 電轉(zhuǎn)印槽Mini-Trans Blot的轉(zhuǎn)移電極芯凝膠容量:2塊Mini-Protean手灌膠,2塊Mini-Protean預(yù)制膠或2塊Ready Gel預(yù)制液
最大凝膠尺寸(寬×長):10×7.5cm
緩沖液要求:450mL
安裝運行條件
相對濕度范圍:0~95% R.H.
工作環(huán)境溫度:0℃~40℃
外觀尺寸(寬×長×高):12×16×18cm
電泳槽重量:1.0kg
(推薦的配套電源PowerPac HC)
組成配置
1、蛋白電轉(zhuǎn)印槽
伯樂Western Blot電轉(zhuǎn)印槽Mini-Trans Blot全套工作配置由緩沖液槽組件(Buffer Tank and Lid)和電轉(zhuǎn)印模塊(Mini Trans-Blot Module)兩部分組成。
電轉(zhuǎn)印模塊(1703935)包含Mini Trans-Blot轉(zhuǎn)移電極芯、轉(zhuǎn)印三明治夾、轉(zhuǎn)印海綿墊、冷卻降溫藍(lán)色冰盒,但不包含緩沖液槽組件。
緩沖液槽組件(1658040)為蛋白跑膠、轉(zhuǎn)膜通用。
Mini-Trans Blot標(biāo)準(zhǔn)配置提供一槽和一模塊,最多可進(jìn)行2塊凝膠的電轉(zhuǎn)膜操作。
如需同時進(jìn)行4塊凝膠轉(zhuǎn)膜,則需額外添置一個電轉(zhuǎn)移模塊與現(xiàn)有蛋白電泳用的緩沖液槽組合,即可進(jìn)行蛋白電轉(zhuǎn)實驗。
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
電轉(zhuǎn)印模塊 | Bio-Rad | 1703935 | 1套 | Mini Trans-Blot Module includes: 2 gel holder cassettes; Foam pads; Electrode assembly; Cooling unit; (does not include lower buffer tank and lid) | 詢價 詢價 |
Mini Trans-Blot 小型電轉(zhuǎn)印槽 | Bio-Rad | ?1703930 | 1套 | Mini Trans-Blot Electrophoretic Transfer Cell for two 10×7.5cm gels includes: Mini Trans-Blot Module (1703935); Buffer Tank and Lid (1658040 | 詢價 詢價 |
2. 蛋白電轉(zhuǎn)槽組件
伯樂Mini-Trans Blot小型電轉(zhuǎn)槽可更換附件主要包括轉(zhuǎn)移電極芯(1703812)、轉(zhuǎn)印三明治夾(1703931)、轉(zhuǎn)印海綿墊(1703933)和輔助工具。
轉(zhuǎn)印海綿墊屬于易損件。
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
Mini Trans-Blot轉(zhuǎn)移電極芯 | Bio-Rad | 1703812 | 1個 | Mini Trans-Blot Central Core assembly module for use with Mini Trans-Blot cell. | 詢價 |
轉(zhuǎn)移三明治夾 | Bio-Rad | 1703931 | 1個 | Mini Gel Holder Cassette,maximum gel size 10×7.5cm for use with Mini Trans-Blot cell. | 詢價 |
轉(zhuǎn)印海綿墊 | Bio-Rad | 1703933 | 4片/包 | replacement pads,8×11cm, for use with Mini Trans-Blot Cell. | 詢價 |
藍(lán)色冷卻盒 | Bio-Rad | 1703919 | 1個 | blue cooling unit use with Mini-Protean Tetra cell tank. | 詢價 |
轉(zhuǎn)印滾筒 | Bio-Rad | 1651279 | 1個 | roller, use with blotting apparatus and whole gel eluter | 詢價 |
基本耗材
伯樂Western Blot電轉(zhuǎn)印槽Mini-Trans Blot的基本耗材,主要包括WB轉(zhuǎn)印膜、轉(zhuǎn)印濾紙等。
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
7.5×10cm厚轉(zhuǎn)印濾紙 | Bio-Rad | 1703932 | 50片/包 | high-wet-strength blot absorbent filter paper for Mini Trans-Blot Cassette 7.5×10cm thick 0.84mm thickness used with semi-dry or wet transfer blotting. | 詢價 |
7×8.4cm加厚轉(zhuǎn)印濾紙 | Bio-Rad | 1703966 | 60片/包 | 7×8.4cm extra thick blot absorbent filter paper 2.45mm thickness for Mini-Protean or Ready Gel Precast Gels used for semi-dry or wet transfer blotting. | 詢價 |
小型PVDF轉(zhuǎn)印膜 | Bio-Rad | 1620174 | 10片/包 | 0.2μm, precut 7×8.4cm Immun-Blot PVDF Membranes for chemiluminescent and colorimetric western blots | 詢價 |
小型PVDF膜-濾紙三明治套裝 | Bio-Rad | 1620219 | 50套/盒 | This Immun-Blot PVDF membrane package contains 50 sheets of 7×8.5cm precut, preassembled blotting membrane and filter paper sandwiches, which fit Ready Gel and Mini-protean precast gels. | 詢價 |
PVDF膜卷筒 | Bio-Rad | 1620177 | 1卷 | Immun-Blot PVDF Membrane Roll 0.2 μm, 26 cm×3.3 m,bulk membrane for high binding (150–160 μg/cm2) immunoblotting | 詢價 |
低熒光 PVDF膜-濾紙三明治套裝 | Bio-Rad | 1620260 | 10套/盒 | This Immun-Blot LF-PVDF membrane package contains 10-sheet, 7×8.5cm precut membrane and 20 sheets of precut filter paper. | 詢價 |
訂貨指南
伯樂Mini-Trans Blot小型濕轉(zhuǎn)槽可容納一個電轉(zhuǎn)印模塊,每輪運行可完成2塊凝膠轉(zhuǎn)膜。視蛋白電泳流程中凝膠的數(shù)量、通量要求,可配置1-2個轉(zhuǎn)移模塊(1703935)以便一輪同時完成2塊或4塊凝膠的轉(zhuǎn)移。
伯樂Mini-Trans Blot電轉(zhuǎn)槽運行需相應(yīng)電泳電源,如常用的Bio-Rad PowerPac Basic基礎(chǔ)型電源(1645050,最大輸出電流400mA,可同時驅(qū)動2塊小型凝膠電轉(zhuǎn))、高電流型電源PowerPacHC(1645052,最大輸出電流3.0A,可同時驅(qū)動4塊小型凝膠電轉(zhuǎn))均可。
根據(jù)實驗室自身條件情況,還可搭配Mini-Protean Tetra蛋白垂直電泳槽,構(gòu)建完整的Western電泳-轉(zhuǎn)膜全流程平臺。
序號 | 品名 | 貨號 | 套裝配置描述 | 報價 |
1-1 | 小型電轉(zhuǎn)印槽 | 1703930 | Mini Trans-Blot Electrophoretic Transfer Cell 配置詳見:“主要附件”部分描述 | 詢價 詢價 |
1-2 | 電源-電轉(zhuǎn)Basic套裝(2膜) | 1703989 | Blot and Power Supply bundle Includes: ■ Mini Trans-Blot Cell (1703930); ■ PowerPac Basic Power Supply (1645050) | 詢價 詢價 |
1-3 | 電源-電轉(zhuǎn)HC套裝(2膜) | 1703836 | Blot and Power Supply bundle includes: ■ Mini Trans-Blot Cell (1703930) ■ PowerPac HC Power Supply (1645052) | 詢價 詢價 |
2-1 | 電泳-轉(zhuǎn)印槽組合裝 (1.0mm/10-well/4-gel/2-blot) | 1658029 | Electrophoresis and blot Bundle (10-well/1.0mm/4-gel) includes: Mini-PROTEAN Tetra Cell system (1658001); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
2-2 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/4-gel/2-blot) | 1658033 | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
2-3 | HC電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/4-gel/2-blot) | 1658035 | Electrophoresis, blot and Power Supply Bundle for 10-well, 1.0 mm, 4-gel system includes: Mini-PROTEAN Tetra Cell (1658001); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052) | 詢價 詢價 |
2-4 | HC電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/4-gel/4-blot) | 1658035-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini Trans-Blot Transfer Cell (1703930); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 詢價 詢價 |
2-5 | HC電源-電泳-轉(zhuǎn)印高級套裝 (1.0mm/10-well/4-gel) (1.5mm/10-well/4-gel) (4-blot) | 1658035-U | Electrophoresis, blot and Power Supply Bundle (10-well/1.0mm/4-gel) includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini-PROTEAN Tetra Cell Casting Module (1658021); Mini Trans-Blot Cell (1703930); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 詢價 詢價 |
3-1 | 電泳-轉(zhuǎn)印槽組合裝 (1.5mm/10-well/2-gel/2-blot) | 16580007-B | Electrophoresis and blot Bundle (10-well/1.5mm/2-gel) includes: Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
3-2 | 電泳-轉(zhuǎn)印組合裝 (1.5mm/10-well/4-gel/2-blot) | 16580006-B | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); | 詢價 詢價 |
3-3 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/2-gel/2-blot) | 16580007-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
3-4 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/4-gel/2-blot) | 16580006-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
3-5 | HC電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/4-gel/4-blot) | 16580006-U | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); Mini Trans-Blot Cell (1703930) PowerPac HC Power Supply (1645052). | 詢價 詢價 |
4-1 | 電泳-轉(zhuǎn)印槽組合裝 (1.0mm/10-well/2-gel) | 1658003-C | Electrophoresis and blot Bundle (10-well/1.0mm/2-gel) includes: Mini-PROTEAN Tetra Cell system (1658003); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
4-2 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/2-gel/2-blot) | 1658003-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
4-3 | 電泳-轉(zhuǎn)印槽組合裝 (1.5mm/10-well/2-gel) | 1658007-C | Electrophoresis and blot Bundle (10-well/1.5mm/2-gel) includes: Mini-PROTEAN Tetra Cell system (1658007); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
4-4 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/2-gel/2-blot) | 1658007-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
5-1 | 蛋白預(yù)制膠 電泳-轉(zhuǎn)印槽組合 | 1658030 | Vertical Electrophoresis and Mini Trans-Blot Module Bundle for Mini Precast 4-Gel includes: 4-gel Mini-PROTEAN Vertical Cell (1658004); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
5-2 | 蛋白預(yù)制膠 Basic電源電泳轉(zhuǎn)印套裝 | 1658034 | Electrophoresis, blot and Power Supply Bundle for precast 4-gel includes: Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
5-3 | 蛋白預(yù)制膠 HC電源電泳轉(zhuǎn)印套裝 | 1658036 | Electrophoresis, blot and Power Supply Bundle includes: for Mini Precast Gels, 4-gel Mini-PROTEAN Tetra Cell (1658004); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 詢價 詢價 |
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