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伯樂Western Blot蛋白電轉(zhuǎn)槽

伯樂Mini-Trans Blot小型蛋白電轉(zhuǎn)印槽

伯樂Mini-Trans Blot 小型蛋白凝膠電轉(zhuǎn)印槽(1703930),即WB電轉(zhuǎn)儀、Western Blot轉(zhuǎn)膜儀。本欄目提供了Mini-Trans Blot 工作組件和常規(guī)Western Blot實驗平臺的選型配置方案指南。

設(shè)備名稱:蛋白電轉(zhuǎn)儀 WB電轉(zhuǎn)印槽 濕式轉(zhuǎn)印槽  轉(zhuǎn)膜槽

制 造 商:美國伯樂(Bio-Rad)公司   產(chǎn)    地:中國

品    牌:伯樂(Bio-Rad)

型    號:Mini-Trans Blot

配置方案:見【訂貨指南】

主要功能用途

蛋白電轉(zhuǎn)印槽,習(xí)稱轉(zhuǎn)膜槽、轉(zhuǎn)膜儀。伯樂Mini Trans-Blot電轉(zhuǎn)印槽可把電泳分離后的蛋白質(zhì)或核酸從聚丙烯酰胺等凝膠中轉(zhuǎn)印至如PVDF膜、硝酸纖維素膜等支持載體上。提供大分子量跨度范圍提供蛋白高效而穩(wěn)定轉(zhuǎn)印。Mini Trans-Blot能同時容納2個小型凝膠轉(zhuǎn)印電極,快速高質(zhì)量地轉(zhuǎn)印2塊小型凝膠,主要用于實驗室蛋白印記分析(Western Blotting)和核酸轉(zhuǎn)印(Northern Bloting\Southern Blotting)分析。

 

性能特點

伯樂Mini-Trans Blot小型電轉(zhuǎn)槽工作時的電壓設(shè)置、轉(zhuǎn)印時間和冷卻方法,均可根據(jù)樣品屬性靈活選擇。

可在1小時內(nèi)完成2塊10×7.5 cm小型凝膠轉(zhuǎn)印,也可以進(jìn)行低強度隔夜轉(zhuǎn)印。

采用電極絲,相距4cm ,產(chǎn)生強電場,產(chǎn)熱少。

彩色標(biāo)記的轉(zhuǎn)印夾和電極確保轉(zhuǎn)印過程中凝膠的正確定向。

藍(lán)色致冷芯可完全置于小型Trans-Blot 轉(zhuǎn)印槽內(nèi),在快速轉(zhuǎn)印過程中吸收熱量。

Mini-Trans Blot電轉(zhuǎn)槽與Mini-PROTEAN Tetra cells (1658001)垂直電泳槽兩套流程,所用的緩沖液槽組件完全兼容,可互換。

 

 

主要技術(shù)規(guī)格

伯樂Western Blot 電轉(zhuǎn)印槽Mini-Trans Blot的轉(zhuǎn)移電極芯凝膠容量:2塊Mini-Protean手灌膠,2塊Mini-Protean預(yù)制膠或2塊Ready Gel預(yù)制液

最大凝膠尺寸(寬×長):10×7.5cm

緩沖液要求:450mL

 

安裝運行條件

相對濕度范圍:0~95% R.H.

工作環(huán)境溫度:0℃~40℃

外觀尺寸(寬×長×高):12×16×18cm

電泳槽重量:1.0kg

推薦的配套電源PowerPac HC

組成配置

1、蛋白電轉(zhuǎn)印槽

伯樂Western Blot電轉(zhuǎn)印槽Mini-Trans Blot全套工作配置由緩沖液槽組件(Buffer Tank and Lid)和電轉(zhuǎn)印模塊(Mini Trans-Blot Module)兩部分組成。
電轉(zhuǎn)印模塊(1703935)包含Mini Trans-Blot轉(zhuǎn)移電極芯、轉(zhuǎn)印三明治夾、轉(zhuǎn)印海綿墊、冷卻降溫藍(lán)色冰盒,但不包含緩沖液槽組件。
緩沖液槽組件(1658040)為蛋白跑膠、轉(zhuǎn)膜通用。
Mini-Trans Blot標(biāo)準(zhǔn)配置提供一槽和一模塊,最多可進(jìn)行2塊凝膠的電轉(zhuǎn)膜操作。
如需同時進(jìn)行4塊凝膠轉(zhuǎn)膜,則需額外添置一個電轉(zhuǎn)移模塊與現(xiàn)有蛋白電泳用的緩沖液槽組合,即可進(jìn)行蛋白電轉(zhuǎn)實驗。

 

品名

品牌

貨號

規(guī)格

描述

報價

電轉(zhuǎn)印模塊

Bio-Rad

1703935

1套

Mini Trans-Blot Module

includes:

2 gel holder cassettes; Foam pads;

Electrode assembly;

Cooling unit;

(does not include lower buffer tank and lid)

詢價

詢價

Mini Trans-Blot 小型電轉(zhuǎn)印槽

Bio-Rad

?1703930

1套

Mini Trans-Blot Electrophoretic Transfer Cell

for two 10×7.5cm gels

includes:

Mini Trans-Blot Module (1703935);

Buffer Tank and Lid (1658040

詢價

詢價

 

 

2. 蛋白電轉(zhuǎn)槽組件

伯樂Mini-Trans Blot小型電轉(zhuǎn)槽可更換附件主要包括轉(zhuǎn)移電極芯(1703812)轉(zhuǎn)印三明治夾(1703931)、轉(zhuǎn)印海綿墊(1703933)輔助工具

轉(zhuǎn)印海綿墊屬于易損件。

品名

品牌

貨號

規(guī)格

描述

報價

Mini Trans-Blot轉(zhuǎn)移電極芯

Bio-Rad

1703812

1個

Mini Trans-Blot Central Core

assembly module for use with Mini Trans-Blot cell.

詢價

轉(zhuǎn)移三明治夾

Bio-Rad

1703931

1個

Mini Gel Holder Cassette,maximum gel size 10×7.5cm

for use with Mini Trans-Blot cell.

詢價

轉(zhuǎn)印海綿墊

Bio-Rad

1703933

4片/包

replacement pads,8×11cm,

for use with Mini Trans-Blot Cell.

詢價

藍(lán)色冷卻盒

Bio-Rad

1703919

1個

blue cooling unit

use with Mini-Protean Tetra cell tank.

詢價

轉(zhuǎn)印滾筒

Bio-Rad

1651279

1個

roller, use with blotting apparatus and whole gel eluter

詢價

 

基本耗材

伯樂Western Blot電轉(zhuǎn)印槽Mini-Trans Blot的基本耗材,主要包括WB轉(zhuǎn)印膜、轉(zhuǎn)印濾紙等。

品名

品牌

貨號

規(guī)格

描述

報價

7.5×10cm厚轉(zhuǎn)印濾紙

Bio-Rad

1703932

50片/包

high-wet-strength blot absorbent filter paper

for Mini Trans-Blot Cassette

7.5×10cm thick

0.84mm thickness

used with semi-dry or wet transfer blotting.

詢價

7×8.4cm加厚轉(zhuǎn)印濾紙

Bio-Rad

1703966

60片/包

7×8.4cm extra thick blot absorbent filter paper

2.45mm thickness

for Mini-Protean or Ready Gel Precast Gels

used for semi-dry or wet transfer blotting.

詢價

小型PVDF轉(zhuǎn)印膜

Bio-Rad

1620174

10片/包

0.2μm, precut 7×8.4cm Immun-Blot PVDF Membranes

for chemiluminescent and colorimetric western blots

詢價

小型PVDF膜-濾紙三明治套裝

Bio-Rad

1620219

50套/盒

This Immun-Blot PVDF membrane package

contains 50 sheets of 7×8.5cm precut,

preassembled blotting membrane

and filter paper sandwiches,

which fit Ready Gel and

Mini-protean precast gels.

詢價

PVDF膜卷筒

Bio-Rad

1620177

1卷

Immun-Blot PVDF Membrane Roll

0.2 μm, 26 cm×3.3 m,bulk membrane

 for high binding (150–160 μg/cm2) immunoblotting

詢價

低熒光 PVDF膜-濾紙三明治套裝

Bio-Rad

1620260

10套/盒

This Immun-Blot LF-PVDF membrane package

contains 10-sheet, 7×8.5cm precut membrane

and 20 sheets of precut filter paper.

詢價



訂貨指南

伯樂Mini-Trans Blot小型濕轉(zhuǎn)槽可容納一個電轉(zhuǎn)印模塊,每輪運行可完成2塊凝膠轉(zhuǎn)膜。視蛋白電泳流程中凝膠的數(shù)量、通量要求,可配置1-2個轉(zhuǎn)移模塊(1703935)以便一輪同時完成2塊或4塊凝膠的轉(zhuǎn)移。

伯樂Mini-Trans Blot電轉(zhuǎn)槽運行需相應(yīng)電泳電源,如常用的Bio-Rad PowerPac Basic基礎(chǔ)型電源(1645050,最大輸出電流400mA,可同時驅(qū)動2塊小型凝膠電轉(zhuǎn))、高電流型電源PowerPacHC(1645052,最大輸出電流3.0A,可同時驅(qū)動4塊小型凝膠電轉(zhuǎn))均可。

根據(jù)實驗室自身條件情況,還可搭配Mini-Protean Tetra蛋白垂直電泳槽,構(gòu)建完整的Western電泳-轉(zhuǎn)膜全流程平臺。

序號

品名

貨號

套裝配置描述

報價

1-1

小型電轉(zhuǎn)印槽

1703930

Mini Trans-Blot   Electrophoretic Transfer Cell

配置詳見:“主要附件”部分描述

詢價

詢價

1-2

電源-電轉(zhuǎn)Basic套裝(2膜)

1703989

Blot and Power Supply bundle

Includes:

■ Mini Trans-Blot Cell (1703930);

■ PowerPac Basic Power Supply (1645050)

詢價

詢價

1-3

電源-電轉(zhuǎn)HC套裝(2膜)

1703836

Blot and Power Supply bundle

includes:

■ Mini Trans-Blot Cell (1703930)

■ PowerPac HC Power Supply (1645052)

詢價

詢價






2-1

電泳-轉(zhuǎn)印槽組合裝

(1.0mm/10-well/4-gel/2-blot)

1658029

Electrophoresis and blot Bundle (10-well/1.0mm/4-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658001);

Mini Trans-Blot Module (1703935).

詢價

詢價

2-2

Basic電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/4-gel/2-blot)

1658033

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

2-3

HC電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/4-gel/2-blot)

1658035

Electrophoresis, blot and Power Supply Bundle

for 10-well, 1.0 mm, 4-gel system

includes:

Mini-PROTEAN Tetra Cell (1658001);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052)

詢價

詢價

2-4

HC電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/4-gel/4-blot)

1658035-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Transfer Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價

2-5

HC電源-電泳-轉(zhuǎn)印高級套裝

(1.0mm/10-well/4-gel)

(1.5mm/10-well/4-gel)

(4-blot)

1658035-U

Electrophoresis, blot and Power Supply Bundle

(10-well/1.0mm/4-gel)

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini-PROTEAN Tetra Cell Casting Module (1658021);

Mini Trans-Blot Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價






3-1

電泳-轉(zhuǎn)印槽組合裝

(1.5mm/10-well/2-gel/2-blot)

16580007-B

Electrophoresis and blot Bundle   (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007);

Mini   Trans-Blot Module (1703935).

詢價

詢價

3-2

電泳-轉(zhuǎn)印組合裝

(1.5mm/10-well/4-gel/2-blot)

16580006-B

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini   Trans-Blot Module (1703935);

詢價

詢價

3-3

Basic電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/2-gel/2-blot)

16580007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac   Basic Power Supply (1645050).

詢價

詢價

3-4

Basic電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/4-gel/2-blot)

16580006-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

3-5

HC電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/4-gel/4-blot)

16580006-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

Mini Trans-Blot Cell (1703930)

PowerPac HC Power Supply (1645052).

詢價

詢價






4-1

電泳-轉(zhuǎn)印槽組合裝

(1.0mm/10-well/2-gel)

1658003-C

Electrophoresis and blot Bundle (10-well/1.0mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658003);

Mini Trans-Blot Module (1703935).

詢價

詢價

4-2

Basic電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/2-gel/2-blot)

1658003-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

4-3

電泳-轉(zhuǎn)印槽組合裝

(1.5mm/10-well/2-gel)

1658007-C

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658007);

Mini Trans-Blot Module (1703935).

詢價

詢價

4-4

Basic電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/2-gel/2-blot)

1658007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價






5-1

蛋白預(yù)制膠

電泳-轉(zhuǎn)印槽組合

1658030

Vertical Electrophoresis and Mini Trans-Blot Module Bundle

for Mini Precast 4-Gel

includes:

4-gel Mini-PROTEAN Vertical Cell (1658004);

Mini Trans-Blot Module (1703935).

詢價

詢價

5-2

蛋白預(yù)制膠

Basic電源電泳轉(zhuǎn)印套裝

1658034

Electrophoresis, blot and Power Supply Bundle

for precast 4-gel

includes:

Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

5-3

蛋白預(yù)制膠

HC電源電泳轉(zhuǎn)印套裝

1658036

Electrophoresis, blot and Power Supply Bundle

includes:

for Mini Precast Gels, 4-gel

Mini-PROTEAN Tetra Cell (1658004);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價

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