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PCR反應(yīng)模塊

96-Well反應(yīng)模塊

樣品通量

96×0.2 mL

樣品體積

1–50μL (10–25μL recommended)

兼容PCR耗材

0.2mL Low-Profile單管

0.2mL Low-Profile Strips

Hard-Shell Low-Profile 96-Well Skirted PCR   Plate

溫度控制

控溫方式

Peltier

熱蓋加熱溫度

最高至110℃

最大升降溫速率

5 ℃/sec

平均升降溫速率

3.3 ℃/sec

溫度控制范圍

0 ～ 100℃

溫度準(zhǔn)確度

±0.2℃ of programmed target at 90℃

溫度均勻性

±0.3℃（well-to-well   after 10 sec of arrival at target 90℃）

梯度溫度設(shè)定范圍

30 ～ 100℃

溫度梯度溫差范圍

1 ～ 24℃

檢測模塊

激發(fā)光源

3個(gè)帶濾光片的LED

熒光激發(fā)范圍

CH1：450-490 nm

CH2：515-535 nm

CH3：450–490 nm

檢測器

3 個(gè)帶濾光片的光敏二極管（photodiodes）

熒光檢測范圍

CH1：波長515-530 nm，F(xiàn)AM/SYBR Green I

CH2：波長560-580 nm, VIC/HEX/CAL Fluor Gold 540/Cal Fluor Orange

CH3：560-580 nm

多重分析功能

2個(gè)目標(biāo)基因

FAM/SYBR Green單通道檢測時(shí)間

3秒

所有通道檢測時(shí)間

12秒

檢測靈敏度

單拷貝人基因組基因

檢測動態(tài)范圍

10數(shù)量級

熔解曲線分辨率

0.1 ℃

CFX Manager Software數(shù)據(jù)分析模式

標(biāo)準(zhǔn)曲線絕對定量

熔解曲線分析、高分辨率熔解曲線

等位基因分型

終點(diǎn)熒光分析

??Cq 基因表達(dá)分析

最適內(nèi)參篩選、帶擴(kuò)增效率校正的多內(nèi)參基因相對定量

（柱形圖、散點(diǎn)圖、火山圖、聚類分析、熱圖）。

聯(lián)機(jī)電腦操作系統(tǒng)

Windows 10 and up

數(shù)據(jù)接口

USB 2.0

質(zhì)量認(rèn)證

IEC，CE

CFX Duet Real-Time PCR Instrument

Bio-Rad

12016265

Each

CFX Duet Real-Time PCR Detection system

96-well, 2-color plus FRET, network-connected

includes CFX Duet base unit, communication cables.

(Order CFX Maestro Software separately)

詢價(jià)

CFX Maestro Software (Windows)

Bio-Rad

12013758

each

CFX Maestro Software Version 2

compatible with Win 7 and 10

includes 1 license for qbase+ Software.

詢價(jià)

CFX Maestro Software (Mac)

Bio-Rad

12004128

each

CFX Maestro Software Version 1.1, Mac Edition

compatible with Mac OS X El Capitan、macOS Sierra

 (does not support instrument control)

詢價(jià)

詢價(jià)

Precision Melt Analysis Software

Bio-Rad

1845015

each

Precision Melt Analysis Software

includes 2 user licenses,

installation CD,

2 HASP HL keys

詢價(jià)

CFX  Opus Wi-Fi Adapter

(China)

Bio-Rad

12013344

each

Wi-Fi antenna enables Wi-Fi connection

for CFX Opus in China

  詢價(jià)

PCR單管蓋管器

Bio-Rad

ECT1000

1個(gè)

Easy Cap Tool,

ensures tight seal for 0.2  mL PCR tubes

or 96-well microplates

詢價(jià)

PCR排管蓋管器

Bio-Rad

ECT2000

1個(gè)

Strip Cap Tool,

for sealing 8-/12-cap  strips on PCR plates or tubes

詢價(jià)

PCR板封膜滾筒

Bio-Rad

MSR0001

1個(gè)

Film Sealing Roller for PCR Plates；

Sealing Roller for sealing PCR plates with films

詢價(jià)

96孔彩色PCR管架

Bio-Rad

TRC0501

5個(gè)/包

96-Place Racks with covers, assorted colors

for PCR tubes;

or unskirted and  semi-skirted microplates.

詢價(jià)

硬框96孔定量PCR板

Bio-Rad

HSL9605

25塊/盒

Hard-Shell 96-Well PCR Plates

low profile, semi-skirted

clear shell/white well PCR plate

詢價(jià)

硬框96孔定量PCR板

（綠邊-白孔）

Bio-Rad

HSL9645

25塊/盒

Hard-Shell 96-Well PCR Plates

low profile, semi-skirted

green shell/white well PCR plate

（discontinued）

詢價(jià)

編碼硬框96孔定量PCR板

Bio-Rad

HSL9905

25塊/盒

Hard-Shell 96-Well PCR Plates

low profile, semi-skirted, clear shell/white

barcoded on 2 sides (Row H and Column 12)

（discontinued）

詢價(jià)

定量PCR封板膜B

Bio-Rad

MSB1001

100張/包

Microseal B Optical Adhesive PCR Plate Sealing Film

詢價(jià)

定量PCR封板膜C

Bio-Rad

MSC1001

100張/包

Microseal C Optical Adhesive PCR Plate Sealing Film

詢價(jià)

白色定量PCR八聯(lián)管

Bio-Rad

TLS0851

120條/盒

0.2ml 8-Tube PCR Strips without Caps

low profile, white, thin-wall

for PCR and low-volume reactions

詢價(jià)

透明PCR   8聯(lián)管

Bio-Rad

TLS0801

120條/盒

0.2ml 8-Tube PCR Strips without Caps

low profile, clear, thin-wall

for PCR and low-volume reactions

詢價(jià)

定量PCR   八聯(lián)平蓋

Bio-Rad

TCS0803

120條/盒

8-cap strips for 0.2ml tubes and plates

for PCR and optical reactions, ultraclear

詢價(jià)

cDNA合成高級試劑盒

Bio-Rad

1725037

個(gè)

iScript Advanced cDNA Synthesis Kit

25 x 20 μl reactions

includes：

100μl iScript Advanced 5x Reaction Mix

25μl iScript Advanced Reverse Transcriptase

詢價(jià)

cDNA合成預(yù)混液

Bio-Rad

1708840

個(gè)

iScript Reverse Transcription Supermix

25 x 20 μl reactions, 100μl

詢價(jià),140

抑制劑耐受型高級

SYBR Green預(yù)混液

Bio-Rad

1725016

個(gè)

SsoAdvanced Universal Inhibitor-Tolerant

SYBR Green Supermix

200 x 20 μl rxns, 2 ml (2 x 1 ml)

詢價(jià)

SYBR Green通用預(yù)混液

Bio-Rad

1725150

個(gè)

iTaq Universal SYBR Green One-Step Kit

100 x 20 μl   reactions, 1 ml

詢價(jià)

SYBR Green通用預(yù)混液

Bio-Rad

1725120

個(gè)

iTaq Universal SYBR Green Supermix

200 x 20μl reactions, 2 ml (2 x 1ml)

詢價(jià)

快速EvaGreen qPCR預(yù)混液 (ROX)

Bio-Rad

1725210

個(gè)

SsoFast EvaGreen Supermix with Low ROX

200 x 20 μl rxns,   2ml (2 x 1.0ml)

詢價(jià)

快速EvaGreen qPCR預(yù)混液

Bio-Rad

1725200

個(gè)

SsoFast EvaGreen Supermix

2x real-time PCR mix

for 200 x 20 μl reactions，2×1ml

詢價(jià)

探針通用預(yù)混液

Bio-Rad

1725140

個(gè)

iTaq Universal Probes One-Step Kit

100 x 20μl reactions, 1ml

詢價(jià)

探針通用預(yù)混液

Bio-Rad

1725130

個(gè)

iTaq Universal Probes Supermix

200 x 20μl reactions, 2ml (2 x 1ml)

詢價(jià)

高級探針預(yù)混液

Bio-Rad

1725280

個(gè)

SsoAdvanced Universal Probes Supermix

200 x 20 μl rxns, 2 ml (2 x 1 ml)

詢價(jià)

高級預(yù)混液

Bio-Rad

1725160

個(gè)

SsoAdvanced PreAmp Supermix

50×50μl rxns, 1.25ml (1×1.25ml)

詢價(jià)

多重定量PCR預(yù)混液

Bio-Rad

1725848

個(gè)

iQ Multiplex Powermix, 1.25ml

50 x 50 μl reactions

詢價(jià)

多重定量PCR用預(yù)混液

Bio-Rad

12010176

EA

Reliance One-Step Multiplex RT-qPCR Supermix

200 x 20 μl reactions

詢價(jià)

1-1

CFX Duet熒光定量PCR儀

入門套裝

12016265-B

CFX Duet Real-Time PCR System with Starter Package

includes:

1）CFX Duet Real-Time PCR Instrument (12016265);

CFX Maestro Software for Windows PC;

license for qbase+ Software;

consumables

2）外置電腦工作站（windows）

3）CFX  Opus Wi-Fi Adapter (China)

詢價(jià)

1-2

CFX Duet熒光定量PCR儀

標(biāo)準(zhǔn)套裝

12016265-S

CFX Duet Real-Time PCR System Research Package

includes:

1）CFX Duet Real-Time PCR System with Starter Package   (12016265-B)

2）PCR體系構(gòu)建工具套件，含：

含PCR單管蓋管器(ECT1000);

PCR排管蓋管器(ECT2000);

PCR板封膜滾筒(MSR0001);

96孔彩色PCR管架(TRC0501)

詢價(jià)