小型蛋白垂直電泳槽
伯樂Mini-Protean Tetra蛋白電泳槽
伯樂Mini-Protean Tetra小型蛋白電泳槽可容納1~4塊小型手灌膠或Ready Gel 預制膠工作,用于蛋白SDS-PAGE垂直電泳分離。本欄目提供Mini-Protean Tetra的組成及選型配置方案。
設備名稱:蛋白垂直電泳槽 蛋白垂直電泳儀
制 造 商:美國Bio-Rad公司 產(chǎn) 地:新加坡/中國
品 牌:伯樂(Bio-Rad)
型 號:Mini-Protean Tetra
電泳配件:見[主要附件] 商品價格:見[訂貨指南]
主要功能用途
Bio-Rad Mini- Protean Tetra蛋白垂直電泳槽,是Western Blot實驗流程重要組成部件,可容納1~4 塊小型手灌膠或Ready Gel 預制膠(10×8.3cm)的電泳,主要用于蛋白聚丙烯酰胺凝膠電泳(PAGE或SDS-PAGE)實驗。
性能特點
伯樂Mini-Protean Tetra蛋白垂直電泳槽,兼容Mini-Protean precast gels預制凝膠、Ready Gel precast gels預制凝膠或手工自制PAGE凝膠。
可在一小時內(nèi)完成多達四塊小型蛋白凝膠的電泳分離。
軟橡膠墊片與玻璃板形成嚴密密封,確保無泄漏灌膠。
帶有簡單凸輪的灌制框,確保在任何平面上實現(xiàn)精確對齊。
平行排列的灌膠架,能同時灌制兩塊凝膠,彈簧杠桿設計使得軟橡膠沉淀產(chǎn)生良好的密封性。
封邊墊條的長玻板加厚,不易破碎。
標有厚度和孔數(shù)的玻板和加樣梳便于識別。
上樣向?qū)Х乐褂镜肋z漏上樣或重復上樣。
可互換模塊可將Mini- Protean Tetra電泳槽轉(zhuǎn)換為可進行Western轉(zhuǎn)印的 Mini Trans-Blot轉(zhuǎn)印槽。
主要技術(shù)規(guī)格
伯樂Mini-Protean Tetra蛋白垂直電泳槽適用的凝膠類型包括:Mini- Protean預制膠、手灌膠
凝膠數(shù):1–4塊
凝膠格式:小型
適用的凝膠尺寸(寬×長)
預制膠:8.6×6.8cm;
手灌膠:8.3×7.3cm
玻璃板尺寸:短板10.1×7.3cm,玻板10.1×8.2cm
緩沖液運行需求
運行2塊凝膠緩沖液總體積:700mL
運行4塊凝膠緩沖液總體積:1000 mL
SDS-PAGE 典型運行時間:35–45 分鐘(在200V恒定電壓下)
Mini-PROTEAN Tetra加樣梳每孔最大上樣量
1.0mm 10孔梳:44 μL
1.0mm 15孔梳:26 μL
安裝運行條件
相對濕度范圍:0~95% R.H.
工作環(huán)境溫度:0℃~40℃
外觀尺寸(寬×長×高):12×16×18cm
Mini- Protean Tetra 電泳槽重量:1.0kg
(電泳電源另配)
組成配置
電泳槽系統(tǒng)
伯樂Mini-Protean Tetra蛋白電泳槽全套工作裝置包括電泳緩沖液槽、電極組件、制膠組件和輔助工具耗材。
按一次可運行的最大凝膠數(shù)量,分為4-gel、2-gel兩種規(guī)格。
如需跑4塊凝膠,可訂購4凝膠槽,也可在2凝膠套裝基礎上加配Mini-Protean Tetra相應功能附件,擴展為4塊凝膠工作通量。
此外,根據(jù)使用的凝膠來源類型,還可單獨配置伯樂預制膠專用套裝。
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
蛋白垂直電泳槽 (4-gel/10-well/1.0mm) | Bio-Rad | 1658001 | 1套 | Mini-PROTEAN Tetra Cell 4-gel system, 1.0mm gel thickness includes: Tank, lid with power cables Mini cell buffer dam; 1×electrode assembly; 1×companion running module(1658038); 2×casting stands, 4 casting frames; 5 sets of short plates and spacer plates; 5×10-well 1.0mm combs 5×gel releasers(1653320) | 詢價 詢價 |
蛋白垂直電泳槽 (4-gel/10-well/1.5mm) | Bio-Rad | 1658006 | 1套 | Mini-PROTEAN Tetra Cell 4-gel system, 1.5mm gel thickness includes: Tank, lid, power cables Mini cell buffer dam; 1×electrode assembly; 1×companion running module(1658038); 2×casting stands, 4 casting frames; 5 sets of short plates and spacer plates; 5×10-well 1.5mm combs 5×gel releasers(1653320) | 詢價 詢價 |
蛋白垂直電泳槽 (4-gel/10-well/0.75mm) | Bio-Rad | 1658000 | 1套 | Mini-PROTEAN Tetra Cell 4-gel system, 0.75mm gel thickness includes: Tank, lid, power cables Mini cell buffer dam; 1×electrode assembly; 1×companion running module(1658038); 2×casting stands, 4 casting frames; 5 sets of short plates and spacer plates; 5×10-well 0.75mm combs 5×gel releasers(1653320) | 詢價 詢價 |
蛋白垂直電泳槽l (2-gel/10-well/1.0mm) | Bio-Rad | 1658003 | 1套 | Mini-PROTEAN Tetra Cell 2-gel system, 1.0mm gel thickness includes: 1×casting stand, 2×casting frames; 5×10-well combs; 5 short plates and 5 spacer plates | 詢價 詢價 |
蛋白垂直電泳槽 (2-gel/10-well/1.5mm) | Bio-Rad | 1658007 | 1套 | Mini-PROTEAN Tetra Cell 2-gel system, 1.5mm gel thickness includes: 1×casting stand, 2×casting frames; 5×10-well combs; 5 sets of glass plates | 詢價 詢價 |
蛋白垂直電泳槽 (2-gel/10-well/0.75mm) | Bio-Rad | 1658002 | 1套 | Mini-PROTEAN Tetra Cell 2-gel system, 0.75 mm gel thickness includes: 1×casting stand, 2×casting frames; 5×10-well combs; 5 short plates and 5 spacer plates | 詢價 詢價 |
Mini Precast Gels(4-gel) 預制膠蛋白電泳槽 | Bio-Rad | 1658004 | 1套 | Mini-PROTEAN Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 4-gel includes: electrode assembly; companion running module; tank, lid with power cables, cell buffer dam | 詢價 詢價 |
Mini Precast Gels (2-gel) 預制膠蛋白電泳槽 | Bio-Rad | 1658005 | 1套 | Mini-PROTEAN Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 2-gel includes: electrode assembly; tank, lid with power cables, cell buffer dam | 詢價 詢價 |
電泳槽組件
伯樂Mini-Protean Tetra 小型蛋白垂直電泳槽的制膠模塊(Casting Module),是指用于灌制特定厚薄和齒孔數(shù)目蛋白凝膠所需的模塊化套裝。
模塊由通用核心組件(Core System)和差異化功能組件組成。通用核心灌膠組件為灌膠架(含4個密封墊),夾膠框(casting frames)和或膠鏟(gel releaser)。差異化功能組件則包括特定規(guī)格的玻璃板套件,電泳梳和上樣導槽。
伯樂Mini-PROTEAN Tetra Cell小型垂直電泳槽有多種灌膠模塊可選,分別滿足不同上樣量和對凝膠厚度的特殊要求。
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
1.0mm/10孔 制膠模塊 | Bio-Rad | 1658015 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 10-well/1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
1.0mm/15孔制膠模塊 | Bio-Rad | 1658016 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 15-well, 1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
1.0mm/5孔 制膠模塊 | Bio-Rad | 1658013 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 5-well, 1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates. | 詢價 詢價 |
1.0mm/9孔 制膠模塊 | Bio-Rad | 1658014 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 9-well, 1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide. | 詢價 詢價 |
1.5mm/10孔 制膠模塊 | Bio-Rad | 1658021 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 10-well, 1.5 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
1.5mm/15孔 制膠模塊 | Bio-Rad | 1658022 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 15-well, 1.5 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
1.5mm/5孔 制膠模塊 | Bio-Rad | 1658019 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 5-well, 1.5 mm gel casting module includes the following componets: 2 casting stands, 4 casting frames 5 combs, 5 short plates and 5 spacer plates. | 詢價 詢價 |
1.5mm/9孔 制膠模塊 | Bio-Rad | 1658020 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 9-well, 1.5 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
0.75mm/10孔 制膠模塊 | Bio-Rad | 1658010 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 10-well, 0.75 mm gel casting module includes the following componets: 2 casting stands, 4 casting frames 5 combs, 5 short plates and 5 spacer plates sample loading guide. | 詢價 詢價 |
0.75mm/15孔 制膠模塊 | Bio-Rad | 1658011 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 15-well, 0.75 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
0.75mm/5孔 制膠模塊 | Bio-Rad | 1658008 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 5-well, 0.75 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
0.75mm/9孔 制膠模塊 | Bio-Rad | 1658009 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 9-well, 0.75 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 詢價 詢價 |
灌膠通用套件(4膠) | Bio-Rad | 1658051 | 1套 | Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit includes: 2×stands, 4×frames,4×gaskets; 5×gel releasers. 適用于4膠Mini-Protean Tetra電泳槽 | 詢價 詢價 |
灌膠通用套件(2膠) | Bio-Rad | 1658052 | 1套 | Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit includes: 1×stand, 2×frames, 2×gaskets; 5×gel releasers. 適用于2膠Mini-Protean Tetra電泳槽 | 詢價 詢價 |
基本耗材
伯樂Mini-Protean Tetra垂直電泳槽工作的基本耗材主要包括蛋白凝膠玻璃板等。
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
1.0mm厚玻璃板 | Bio-Rad | 1653311 | 5塊/包 | Spacer Plates with 1.0 mm Integrated Spacers 帶1.0mm厚封邊條,為1.0mm凝膠灌膠組件。 | 詢價 |
1.5mm厚玻璃板 | Bio-Rad | 1653312 | 5塊/包 | spacer plate with 1.5 mm integrated spacer 帶1.5mm厚封邊條,用于1.5mm凝膠的制備 | 詢價 |
薄玻璃板 | Bio-Rad | 1653308 | 5塊/包 | short plate for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber. 外形尺寸(W×L):101× 73 mm 與帶任一厚度封邊墊條的長玻璃板配套使用 | 詢價 |
訂貨指南
1、蛋白電泳槽的選型
因不同實驗樣品樣品數(shù)量、蛋白類型和表達豐度、實驗經(jīng)驗與操作習慣的不同,選擇蛋白電泳槽重點要結(jié)合所用凝膠的厚度(0.75mm、1.0mm、1.5mm)、每張凝膠電泳的上樣孔數(shù)(常用的10孔-最大上樣量66μl、15孔-40μl)、每輪電泳的凝膠通量(4膠、2膠)和凝膠類型(手灌膠、預制膠)來確定具體選型方案。
如單獨訂購Mini-Protean Tetra小型蛋白垂直電泳槽或更換電泳灌膠配件,請請參考本欄目“組成配置”“主機”部分所列各型電泳槽的技術(shù)規(guī)格和詳細配置內(nèi)容。最常用的1658001槽單元,其標準配置適用的是1.0mm/10-well/4-gel凝膠。
2、蛋白電泳槽電源的選擇
伯樂Mini-Protean Tetra小型蛋白垂直電泳槽本身工作運行對電源并無特殊要求。伯樂PowerPac Basic 、PowerPac HC、PowerPac Universal等多個型號均可滿足同時給1-4個電泳槽供電的要求。
但須注意的是,通常情況下,蛋白電泳槽只是Western Blot實驗流程的一個功能組件,單個電泳槽輸出凝膠數(shù)量(2塊或4塊)與電轉(zhuǎn)印槽(2塊)各自最大凝膠工作通量不同,要充分考慮實驗流程上的有效銜接問題。
特別是當實驗樣品數(shù)量多于4塊凝膠的情況下,根據(jù)自身實驗條件和經(jīng)驗,通盤考慮電轉(zhuǎn)印槽、電轉(zhuǎn)印模塊的配置數(shù)量。
與常規(guī)140V左右恒壓蛋白電泳跑膠不同,電轉(zhuǎn)印對電流要求較高。通常,每塊凝膠-印跡膜的工作電流可達200mA甚至更高。若同時進行4塊凝膠轉(zhuǎn)膜操作,單一Basic電源很可能無法滿足供電要求。故需考慮HC、PowerPac Universal等具有電流輸出能力大于0.8A的電源型號確有必要。
3、電泳槽與與Mini-Trans Blot小型蛋白電泳轉(zhuǎn)印槽搭配
電轉(zhuǎn)移槽的選型配置問題,請參考Mini-Trans Blot小型蛋白轉(zhuǎn)印槽欄目內(nèi)容。
序號 | 品名 | 貨號 | 套裝配置描述 | 報價 |
1-1 | Basic蛋白電泳套裝 (1.0mm/10-well/4-gel) | 1658025 | Mini-PROTEAN Tetra and Power Supply Bundle includes: Mini-PROTEAN Tetra 4-gel system (1658001); PowerPac Basic power supply (1645050). | 詢價 詢價 |
1-2 | HC蛋白電泳套裝 (1.0mm/10-well/4-gel) | 1658027 | Mini-PROTEAN Tetra and Power Supply Bundle includes: Mini-PROTEAN Tetra 4-gel system (1658001); PowerPac HC power supply (1645052). | 詢價 詢價 |
2-1 | 電泳-轉(zhuǎn)印槽組合裝 (1.0mm/10-well/4-gel/2-blot) | 1658029 | Electrophoresis and blot Bundle (10-well/1.0mm/4-gel) includes: Mini-PROTEAN Tetra Cell system (1658001); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
2-2 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/4-gel/2-blot) | 1658033 | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
2-3 | HC電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/4-gel/2-blot) | 1658035 | Electrophoresis, blot and Power Supply Bundle for 10-well, 1.0 mm, 4-gel system includes: Mini-PROTEAN Tetra Cell (1658001); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052) | 詢價 詢價 |
2-4 | HC電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/4-gel/4-blot) | 1658035-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini Trans-Blot Transfer Cell (1703930); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 詢價 詢價 |
2-5 | HC電源-電泳-轉(zhuǎn)印高級套裝 (1.0mm/10-well/4-gel) (1.5mm/10-well/4-gel) (4-blot) | 1658035-U | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini-PROTEAN Tetra Cell Casting Module (1658021); Mini Trans-Blot Cell (1703930); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 詢價 詢價 |
3-1 | 電泳-轉(zhuǎn)印槽組合裝 (1.5mm/10-well/2-gel/2-blot) | 16580007-B | Electrophoresis and blot Bundle (10-well/1.5mm/2-gel) includes: Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
3-2 | 電泳-轉(zhuǎn)印組合裝 (1.5mm/10-well/4-gel/2-blot) | 16580006-B | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); | 詢價 詢價 |
3-3 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/2-gel/2-blot) | 16580007-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
3-4 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/4-gel/2-blot) | 16580006-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEA Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
3-5 | HC電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/4-gel/4-blot) | 16580006-U | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); Mini Trans-Blot Cell (1703930) PowerPac HC Power Supply (1645052). | 詢價 詢價 |
4-1 | 電泳-轉(zhuǎn)印槽組合裝 (1.0mm/10-well/2-gel) | 1658003-C | Electrophoresis and blot Bundle (10-well/1.0mm/2-gel) includes: Mini-PROTEAN Tetra Cell system (1658003); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
4-2 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.0mm/10-well/2-gel/2-blot) | 1658003-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
4-3 | 電泳-轉(zhuǎn)印槽組合裝 (1.5mm/10-well/2-gel) | 1658007-C | Electrophoresis and blot Bundle (10-well/1.5mm/2-gel) includes: Mini-PROTEAN Tetra Cell system (1658007); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
4-4 | Basic電源-電泳-轉(zhuǎn)印套裝 (1.5mm/10-well/2-gel/2-blot) | 1658007-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
5-1 | 蛋白預制膠 電泳-轉(zhuǎn)印槽組合 | 1658030 | Vertical Electrophoresis and Mini Trans-Blot Module Bundle for Mini Precast 4-Gel includes: 4-gel Mini-PROTEAN Vertical Cell (1658004); Mini Trans-Blot Module (1703935). | 詢價 詢價 |
5-2 | 蛋白預制膠 Basic電源電泳轉(zhuǎn)印套裝 | 1658034 | Electrophoresis, blot and Power Supply Bundle for precast 4-gel includes: Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 詢價 詢價 |
5-3 | 蛋白預制膠 HC電源電泳轉(zhuǎn)印套裝 | 1658036 | Electrophoresis, blot and Power Supply Bundle includes: for Mini Precast Gels, 4-gel Mini-PROTEAN Tetra Cell (1658004); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 詢價 詢價 |
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