Optima超速離心機轉(zhuǎn)頭適配離心管
Optima XE-100/90超速離心機VTi 90 VTi 65.2垂直轉(zhuǎn)頭
VTi 90、VTi 65.2垂直轉(zhuǎn)頭離心2.0mL g-MAX管、4.9mL指封管及5.1mL熱封管,常用于質(zhì)粒DNA純化或蛋白質(zhì)的密度梯度離心。適用于Optima XPN-100/XPN-90/XPN-80和Optima XE-100/XE-90系列超速離心機。
商品名稱:VTi 90 VTi 65.2垂直轉(zhuǎn)頭 Vertical-Tube Rotor
制 造 商:美國貝克曼庫爾特(Beckman Coulter)公司
品 牌:Beckman
轉(zhuǎn)頭配套適配器、離心管見:[訂購信息]
詳細描述
VTi 90、VTi 65.2垂直轉(zhuǎn)頭由鈦合金制成,可離心2.0 – 5.1mL樣品管,常用于質(zhì)粒DNA純化或蛋白質(zhì)的密度梯度離心。適用于貝克曼庫爾特高端Optima XPN-100/XPN-90/XPN-80型立式離心機和基礎型Optima XE-100/XE-90超速離心機。
1、轉(zhuǎn)頭組件
轉(zhuǎn)頭由鈦合金制成,并涂有黑色聚氨酯(polyurethane)漆。
Quick-Seal和OptiSeal兩款離心管,需添加管蓋狀的管帽墊片/墊片(tube spacer)對離心管上部提供支撐,避免在強大水平離心力作用下,離心管上部變形。黑色適配器用于Quick-Seal熱封管,金色適配器用于OptiSeal管。
超速離心機運行中,為避免因離心管內(nèi)殘留少量空氣因直接暴露于離心腔體的真空環(huán)境而過度膨脹、離心管變形引發(fā)樣品泄漏風險,需將離心孔內(nèi)的氣壓維持在實驗室正常氣壓水平。轉(zhuǎn)頭標配的黑色陽極氧化鋁材質(zhì)六邊形轉(zhuǎn)頭塞(hex-cavity rotor plug)與密封墊圈(plug gasket),用于有效密封離心孔,將離心管與離心腔真空環(huán)境有效隔離。
為了防止超速,超速離心機中的光電探測器會監(jiān)測轉(zhuǎn)頭底部的超速盤,如果檢測到速度超過額度轉(zhuǎn)速,則自動中止運行。
2、轉(zhuǎn)頭準備(Rotor Preparation)
在非室溫條件的離心,需事先在冰箱中預冷或恒溫箱體內(nèi)將轉(zhuǎn)頭加熱轉(zhuǎn)頭到指定溫度備用。
確保轉(zhuǎn)頭塞螺紋清潔,并用潤滑劑 (306812) 均勻潤滑,以最大限度地減少螺紋摩損,確保有效密封。
將轉(zhuǎn)頭固定在轉(zhuǎn)頭鉗臺(rotor vise342705)中,鉗臺應用螺栓固定或夾緊在剛性操作臺面上。
將已填充的樣品管、連同正確的適配器墊片、轉(zhuǎn)頭塞對稱地裝入轉(zhuǎn)頭。樣品管裝入離心孔后,還應在管上方添加管帽墊片墊片(和浮置墊片,如果適用的話)完成裝載。
每個離心管適配器墊片上插入一個轉(zhuǎn)頭塞(墊圈端朝下),使用 T40 Torx轉(zhuǎn)頭塞配套適配器(T40 Torx adapter),用扭矩扳手(torque wrench 369791)擰緊塞的同時,向下按壓六角轉(zhuǎn)頭塞適配器以免打滑。將每個轉(zhuǎn)頭塞擰緊至 13.6 N?m,使轉(zhuǎn)頭塞的頂面略低于轉(zhuǎn)頭表面即可。
3、樣品取出回收(Removal and Sample Recovery)
將VTi 65.2垂直轉(zhuǎn)頭轉(zhuǎn)頭從離心機垂直向上托起離開驅(qū)動軸取出后,重新固定于鉗臺,并用扭矩扳手和轉(zhuǎn)頭塞適配器解封離心孔。
使用適當?shù)牟鹦豆ぞ咭瞥龎|片和管子。
4、轉(zhuǎn)頭技術規(guī)格
轉(zhuǎn)頭型號 | VTi 90 | VTi 65.2 |
最高轉(zhuǎn)速 | 90000 rpm | 65000 rpm |
最高轉(zhuǎn)速時溶液額定密度 (Density rating at maximum speed) | 1.7 g/mL | |
最高轉(zhuǎn)速時相對離心場(Relative Centrifugal Field) | ||
最大離心半徑/最大離心力 | Rmax (71.1 mm)/645000×g | Rmax (87.9 mm)/416000×g |
平均離心半徑/平均離心力 | Rav (64.5 mm)/585000×g | Rav (81.3 mm)/385000×g |
最小離心半徑/最小離心力 | Rmin (57.9 mm)/525000×g | Rmin (74.7 mm)/353000×g |
最大速度下 k 系數(shù)(k factor) | 6 | 10 |
額定離心管尺寸 (Nominal tube dimensions) | 13×51 mm | |
額定離心容量(Nominal tube capacity) | 8×5.1 mL | 16×5.1mL |
達到最大速度的近似加速時間(滿載) | 9.0 min | 11.0 min |
從最大速度(滿載)開始的減速時間 | 8.5 min | 8.0 min |
滿載轉(zhuǎn)頭重量 | 5.5 kg | 8.5 kg |
轉(zhuǎn)頭的材質(zhì) | 鈦合金 |
5、轉(zhuǎn)頭適配離心管
VTi 90 和VTi 65.2垂直轉(zhuǎn)頭的一次性指封管和熱封管(標準配置不含離心管,需單獨訂購)可通用。
為實現(xiàn)離心管有效密封,Quick-Seal管的密封需采用管蓋熱密封器(Cordless Tube Topper)工作套件操作。
熱封管離心結(jié)束后,需使用浮置墊片提取工具(Floating spacer removal tool 338765)、離心管提取工具(Tube Removal Tool, 361668),從離心孔中取出管帽墊片(spacers)和離心管。
訂購信息
品名 | 品牌 | 貨號 | 規(guī)格 | 描述 | 報價 |
VTi 90垂直轉(zhuǎn)頭套件 | Beckman | 362751 | set | VTi 90 Vertical-Tube Rotor Package Standard accessories includes: 1x 342705 Rotor Vise 1x 858121 Torque Wrench 1x 976959 Torx Wrench Adapter 1x 306812 Spinkote Lubricant 2x 355539 Over-speed Disk 12x 342882 Rotor Plug Gaskets | 詢價 詢價 |
5.1mL熱封管工作套裝 | Beckman | 360979 | set | 5.1mL Polypropylene Quick-Seal Tube Kit Applicable Ultracentrifuge Rotor: VTi 90/NVT 90 includes: 4盒 342412 Quick-Seal Polypropylene Tubes 8個 342883 Spacers 1個 348122 Tube Rack 1個 361668 Tube Extraction Tool Cordless Tube Topper Kit required | 詢價 詢價 |
4.9mL指封管工作套裝 | Beckman | 360970 | set | 4.9mL Polypropylene OptiSeal Tube Kit Applicable Ultracentrifuge Rotor: VTi 90/NVT 90 includes: 4盒 362185 OptiSeal Polypropylene Tubes 8個 362198 Spacers 1個 361638 Tube Rack 1個 361668 Tube Extraction Tool 1個 338765 Spacer Removal Tool | 詢價 詢價 |
2mL g-Max管工作套裝 | Beckman | 357325 | set | 2 mL Quick-Seal Polypropylene Tube Kit Applicable Ultracentrifuge Rotor: VTi 90/NVT 90 includes: 50個 2mL g-Max快封管 345829 8個 管帽適配器 342883 1個 g-Max取管鑷子 878446 1個 取帽工具 338765 | 詢價 詢價 |
VTi 65.2垂直轉(zhuǎn)頭套件 | Beckman | 362754 | set | VTi 65.2 Vertical-Tube Rotor Package Standard accessories includes: 1x 342705 Rotor Vise 1x 858121 Torque Wrench 1x 976959 Torx Wrench Adapter 1x 306812 Spinkote Lubricant 2x 330338 Over-speed Disks 12x 342882 Rotor Plug Gaskets | 詢價 詢價 |
5.1mL熱封管工作套裝 | Beckman | 360977 | set | 5.1mL Polypropylene Quick-Seal Tube Kit Applicable Ultracentrifuge Rotor: NVT 65.2/VTi 65.2 includes: 4盒 342412 Quick-Seal Polypropylene Tubes 16個 342883 Spacers 1個 348122 Tube Rack 1個 361668 Tube Extraction Tool (Cordless Tube Topper Kit required) | 詢價 詢價 |
4.9mL指封管工作套裝B | Beckman | 360972 | set | 4.9mL Polypropylene OptiSeal Tube Kit Applicable Ultracentrifuge Rotor: NVT 65.2/VTi 65.2 includes: 4盒 362185 OptiSeal Polypropylene Tubes 16個 362198 Spacers 1個 361638 Tube Rack 1個 361668 Tube Extraction Tool 1個 338765 Spacer Removal Tool | 詢價 詢價 |
2mL g-Max管工作套裝 | Beckman | 357326 | set | 2 mL Quick-Seal Polypropylene Tube Kit Applicable Ultracentrifuge Rotor: NVT 65.2/VTi 65.2 includes: 50個 2mL g-Max快封管 345829 16個 管帽適配器 342883 1個 g-Max取管鑷子 878446 1個 取帽工具 338765 | 詢價 詢價 |
5.1mL超凈熱封管 | Beckman | 344075 | 50/pkg | 5.1mL Quick-Seal Round-Top Ultra-Clear Tube Nominal Size: 13×51mm - 50Pk Applicable Ultracentrifuge Rotor: NVT 90, NVT 65.0 Required Quick-Seal Tube Spacer: 342883 | 詢價 詢價 |
5.1mL熱封管 | Beckman | 342412 | 50/pkg | 5.1mL Quick-Seal Round-Top Polypropylene Tube Nominal Size: 13 x 51mm - 50Pk | 詢價 詢價 |
Φ13mm管帽墊片 | Beckman | 342883 | 1/pkg | 13mm Diameter Aluminum Tube Spacer | 詢價 詢價 |
Φ13mm離心管架 | Beckman | 348122 | 1/pkg | Tube Rack for 13.0mm Diameter Tubes Applicable Ultracentrifuge Rotor: TLA-110, TLA-100.3,TLN-100 | 詢價 詢價 |
2mL熱封管 | Beckman | 345829 | 50/pkg | 2mL Quick-Seal g-max Polypropylene Tube Nominal Size: 13×25mm - 50Pk Applicable Ultracentrifuge Rotor: Type 100 T, Type 50.4 Ti Required Spacer: 360270 | 詢價 詢價 |
Φ13mm管帽墊片 | Beckman | 342883 | 1/pkg | 13mm Diameter Aluminum Tube Spacer | 詢價 詢價 |
Φ13mm浮置墊片 | Beckman | 345827 | 1Pk | 13mm Diameter Noryl Bell-Top Tube Floating Spacer | 詢價 詢價 |
Φ13mm離心管架 | Beckman | 348122 | 1/pkg | Tube Rack for 13.0mm Diameter Tubes Applicable Ultracentrifuge Rotor: TLA-110, TLA-100.3,TLN-100 | 詢價 詢價 |
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